Journal: Journal of Personalized Medicine

Article Title: A New Versatile Platform for Assessment of Improved Cardiac Performance in Human-Engineered Heart Tissues

doi: 10.3390/jpm12020214

Figure Lengend Snippet: Morphological analysis of EHTs in four different media at day 21 of culture. ( A ) Brightfield images of full EHTs in BPEL+HS, DMEM+HS, MM+HS, or MM(SF). Scale bar = 0.5 mm. ( B ) Confocal images of whole-mount tissue immunostaining for cardiac troponin T (cTnT, red) counterstained with DAPI (nuclei, blue). Scale bars, 25 µm. HS, horse serum and SF, serum-free.

Article Snippet: Primary antibody anti-cardiac troponin T (1:400; Invitrogen, MA5-12960), anti-connexin-43 (1:200; Sigma-Aldrich, C6219), or anti-cardiac troponin I3 (1:800; Abcam, ab10231) were then incubated for 2 days at 4 °C.

Techniques: Immunostaining

Journal: Neurotoxicology

Article Title: Molecular stress response in the CNS of mice after systemic exposure to interferon-alpha, ionizing radiation and ketamine.

doi: 10.1016/j.neuro.2008.12.012

Figure Lengend Snippet: Fig. 4. Comparison RNA and protein expression of Tnnt1 in mouse brain treated with ketamine. (A) Fluorescence immunohistochemistry staining. Fluorescence red cytoplasm staining indicated Tnnt 1 protein expression. (B) RNA in situ hybridization. Brownish cytoplasm staining indicated Tnnt 1 RNA expression. The indication of arrow’s color: white: Purkinje cells; yellow: pyramidal cells; red: hippocampus. *Data from Lowe et al. (2007) (magnification: 40). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of the article.)

Article Snippet: Briefly, goat anti-mouse troponin T1 (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) at 1:50 dilution was used as primary antibody and was incubated with sections at 4 8C over night.

Techniques: Comparison, Expressing, Fluorescence, Immunohistochemistry, Staining, RNA In Situ Hybridization, RNA Expression

Journal: Scientific Reports

Article Title: Elucidating the involvement of apoptosis in postmortem proteolysis in porcine muscles from two production cycles using metabolomics approach

doi: 10.1038/s41598-021-82929-3

Figure Lengend Snippet: Effect of different production replicates and muscle types on proteolysis and apoptosis features of porcine skeletal muscles over 7-day postmortem aging.

Article Snippet: After being washed 3 times for 10 min with PBST solution, membranes were incubated with the following secondary antibody preparations for one hour at room temperature: goat anti-mouse IgG (H + L) horseradish peroxidase conjugate (dilution of 1:20,000 for desmin, troponin T, and calpain-1; dilution of 1:5000 for HSP27; Bio-Rad) or goat anti-rabbit IgG peroxidase conjugated (dilution of 1:5000 for cytochrome c and αβ-crystallin, ThermoFisher Scientific).

Techniques: